Plasmid

Part:BBa_K3839043:Design

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-21)


pcDNA3.4-TOPO + CYP2C19_201


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1598
    Illegal XbaI site found at 723
    Illegal SpeI site found at 64
    Illegal PstI site found at 1626
    Illegal PstI site found at 4204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1598
    Illegal SpeI site found at 64
    Illegal PstI site found at 1626
    Illegal PstI site found at 4204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1598
    Illegal BglII site found at 3154
    Illegal BglII site found at 7336
    Illegal BamHI site found at 760
    Illegal BamHI site found at 1392
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1598
    Illegal XbaI site found at 723
    Illegal SpeI site found at 64
    Illegal PstI site found at 1626
    Illegal PstI site found at 4204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1598
    Illegal XbaI site found at 723
    Illegal SpeI site found at 64
    Illegal PstI site found at 1626
    Illegal PstI site found at 4204
    Illegal NgoMIV site found at 2797
    Illegal NgoMIV site found at 3314
    Illegal NgoMIV site found at 4655
    Illegal NgoMIV site found at 4940
    Illegal AgeI site found at 2255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3037
    Illegal BsaI.rc site found at 6468
    Illegal SapI site found at 5385
    Illegal SapI.rc site found at 4504
    Illegal SapI.rc site found at 4714


Design Notes

HTML img Tag



References